Rab3a controls exocytosis in cholecystokinin-secreting cells
Détails
ID Serval
serval:BIB_51411913FB25
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Rab3a controls exocytosis in cholecystokinin-secreting cells
Périodique
FEBS Letters
ISSN
0014-5793 (Print)
Statut éditorial
Publié
Date de publication
08/2001
Volume
503
Numéro
1
Pages
19-24
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 10
Research Support, Non-U.S. Gov't --- Old month value: Aug 10
Résumé
The expression of rab3A and rab3D isoforms in the enteroendocrine, cholecystokinin-secreting, cell lines STC-1 and GLUTag is here demonstrated. In contrast, rab3B is undetectable in these two cell lines, and rab3C is only slightly expressed in GLUTag cells. Using a transient co-transfection system with human growth hormone as reporter protein, we show that overexpression of the GTPase-deficient mutant rab3AQ81L, but not rab3DQ81L, significantly decreases human growth hormone secretory responses to various agonists in STC-1 cells. These results indicate that endocrine cell lines of intestinal origin express rab3A and rab3D proteins, but the GTP-bound form of rab3A only acts as a negative modulator in the control of cholecystokinin secretion from STC-1 cells.
Mots-clé
Animals
Cell Line
Cholecystokinin/*secretion
Exocytosis/*physiology
Genes, Reporter
Growth Hormone/genetics
Immunohistochemistry
Mice
Mutation
Rats
Transfection
rab3A GTP-Binding Protein/genetics/*physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:29
Dernière modification de la notice
20/08/2019 15:07