Rab3a controls exocytosis in cholecystokinin-secreting cells

Details

Serval ID
serval:BIB_51411913FB25
Type
Article: article from journal or magazin.
Collection
Publications
Title
Rab3a controls exocytosis in cholecystokinin-secreting cells
Journal
FEBS Letters
Author(s)
Gevrey  J. C., Laurent  S., Saurin  J. C., Nemoz-Gaillard  E., Regazzi  R., Chevrier  A. M., Chayvialle  J. A., Abello  J.
ISSN
0014-5793 (Print)
Publication state
Published
Issued date
08/2001
Volume
503
Number
1
Pages
19-24
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 10
Abstract
The expression of rab3A and rab3D isoforms in the enteroendocrine, cholecystokinin-secreting, cell lines STC-1 and GLUTag is here demonstrated. In contrast, rab3B is undetectable in these two cell lines, and rab3C is only slightly expressed in GLUTag cells. Using a transient co-transfection system with human growth hormone as reporter protein, we show that overexpression of the GTPase-deficient mutant rab3AQ81L, but not rab3DQ81L, significantly decreases human growth hormone secretory responses to various agonists in STC-1 cells. These results indicate that endocrine cell lines of intestinal origin express rab3A and rab3D proteins, but the GTP-bound form of rab3A only acts as a negative modulator in the control of cholecystokinin secretion from STC-1 cells.
Keywords
Animals Cell Line Cholecystokinin/*secretion Exocytosis/*physiology Genes, Reporter Growth Hormone/genetics Immunohistochemistry Mice Mutation Rats Transfection rab3A GTP-Binding Protein/genetics/*physiology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:29
Last modification date
20/08/2019 15:07
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