Identification and antifungal susceptibility of a large collection of yeast strains isolated in Tunisian hospitals.

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Serval ID
serval:BIB_5138A68E0E45
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification and antifungal susceptibility of a large collection of yeast strains isolated in Tunisian hospitals.
Journal
Medical Mycology
Author(s)
Eddouzi J., Lohberger A., Vogne C., Manai M., Sanglard D.
ISSN
1460-2709 (Electronic)
ISSN-L
1369-3786
Publication state
Published
Issued date
2013
Volume
51
Number
7
Pages
737-746
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
Abstract In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used as a rapid method to identify yeasts isolated from patients in Tunisian hospitals. When identification could not be exstablished with this procedure, sequencing of the internal transcribed spacer with 5.8S ribosomal DNA (rDNA) (ITS1-5.8S-ITS2) and D1/D2 domain of large-subunit (LSU rDNA) were employed as a molecular approach for species differentiation. Candida albicans was the dominant species (43.37% of all cases), followed by C. glabrata (16.55%), C. parapsilosis (13.23%), C. tropicalis (11.34%), C. dubliniensis (4.96%), and other species more rarely encountered in human diseases such as C. krusei, C. metapsilosis, C. lusitaniae, C. kefyr, C. palmioleophila, C. guilliermondii, C. intermedia, C. orthopsilosis, and C. utilis. In addition, other yeast species were obtained including Saccharomyces cerevisiae, Debaryomyces hansenii (anamorph known as C. famata), Hanseniaspora opuntiae, Kodamaea ohmeri, Pichia caribbica (anamorph known as C. fermentati), Trichosporon spp. and finally a novel yeast species, C. tunisiensis. The in vitro antifungal activities of fluconazole and voriconazole were determined by the agar disk diffusion test and Etest, while the susceptibility to additional antifungal agents was determined with the Sensititre YeastOne system. Our results showed low incidence of azole resistance in C. albicans (0.54%), C. tropicalis (2.08%) and C. glabrata (4.28%). In addition, caspofungin was active against most isolates of the collection with the exception of two K. ohmeri isolates. This is the first report to describe caspofungin resistant isolates of this yeast.
Pubmed
Web of science
Open Access
Yes
Create date
17/10/2013 16:32
Last modification date
14/02/2022 7:55
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