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An apparatus for determination of kinetic parameters associated with immobilized enzymes
The advantages associated with the described immobilized enzyme reactor include: (1) Use of the common rate equations and kinetic parameters. (2) Detection of significant lag periods. (3) Quantitative measure for non-covalently attached enzyme. (4) The means for washing the immobilized enzyme allows for the repeated use of the same matrix-bound enzyme. (5) Constant temperature control. (6) Both unbound native and matrix-bound enzyme may be reacted under identical conditions. (7) No grinding of the glass-bound enzyme or other matrix fragmentation occurs because no abrasive forces are required for stirring.
Animals, Chickens, Glass, *Kinetics, L-Lactate Dehydrogenase/*metabolism, Methods, Myocardium/enzymology, Solubility, Spectrophotometry, Ultraviolet, Temperature, Time Factors, Trypsin/*metabolism
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