An apparatus for determination of kinetic parameters associated with immobilized enzymes

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_503B22DADE5B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An apparatus for determination of kinetic parameters associated with immobilized enzymes
Périodique
Analytical Biochemistry
Auteur(s)
Widmer F., Dixon J.E., Kaplan N.O.
ISSN
0003-2697
Statut éditorial
Publié
Date de publication
1973
Peer-reviewed
Oui
Volume
55
Numéro
1
Pages
282-287
Langue
anglais
Résumé
The advantages associated with the described immobilized enzyme reactor include: (1) Use of the common rate equations and kinetic parameters. (2) Detection of significant lag periods. (3) Quantitative measure for non-covalently attached enzyme. (4) The means for washing the immobilized enzyme allows for the repeated use of the same matrix-bound enzyme. (5) Constant temperature control. (6) Both unbound native and matrix-bound enzyme may be reacted under identical conditions. (7) No grinding of the glass-bound enzyme or other matrix fragmentation occurs because no abrasive forces are required for stirring.
Mots-clé
Animals, Chickens, Glass, *Kinetics, L-Lactate Dehydrogenase/*metabolism, Methods, Myocardium/enzymology, Solubility, Spectrophotometry, Ultraviolet, Temperature, Time Factors, Trypsin/*metabolism
Pubmed
Web of science
Création de la notice
13/08/2015 8:53
Dernière modification de la notice
03/03/2018 17:08
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