An apparatus for determination of kinetic parameters associated with immobilized enzymes
Détails
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Accès restreint UNIL
Etat: Public
Version: de l'auteur⸱e
Accès restreint UNIL
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_503B22DADE5B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
An apparatus for determination of kinetic parameters associated with immobilized enzymes
Périodique
Analytical Biochemistry
ISSN
0003-2697
Statut éditorial
Publié
Date de publication
1973
Peer-reviewed
Oui
Volume
55
Numéro
1
Pages
282-287
Langue
anglais
Résumé
The advantages associated with the described immobilized enzyme reactor include: (1) Use of the common rate equations and kinetic parameters. (2) Detection of significant lag periods. (3) Quantitative measure for non-covalently attached enzyme. (4) The means for washing the immobilized enzyme allows for the repeated use of the same matrix-bound enzyme. (5) Constant temperature control. (6) Both unbound native and matrix-bound enzyme may be reacted under identical conditions. (7) No grinding of the glass-bound enzyme or other matrix fragmentation occurs because no abrasive forces are required for stirring.
Mots-clé
Animals, Chickens, Glass, *Kinetics, L-Lactate Dehydrogenase/*metabolism, Methods, Myocardium/enzymology, Solubility, Spectrophotometry, Ultraviolet, Temperature, Time Factors, Trypsin/*metabolism
Pubmed
Web of science
Création de la notice
13/08/2015 7:53
Dernière modification de la notice
20/08/2019 14:06