An apparatus for determination of kinetic parameters associated with immobilized enzymes

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State: Public
Version: author
Serval ID
serval:BIB_503B22DADE5B
Type
Article: article from journal or magazin.
Collection
Publications
Title
An apparatus for determination of kinetic parameters associated with immobilized enzymes
Journal
Analytical Biochemistry
Author(s)
Widmer F., Dixon J.E., Kaplan N.O.
ISSN
0003-2697
Publication state
Published
Issued date
1973
Peer-reviewed
Oui
Volume
55
Number
1
Pages
282-287
Language
english
Abstract
The advantages associated with the described immobilized enzyme reactor include: (1) Use of the common rate equations and kinetic parameters. (2) Detection of significant lag periods. (3) Quantitative measure for non-covalently attached enzyme. (4) The means for washing the immobilized enzyme allows for the repeated use of the same matrix-bound enzyme. (5) Constant temperature control. (6) Both unbound native and matrix-bound enzyme may be reacted under identical conditions. (7) No grinding of the glass-bound enzyme or other matrix fragmentation occurs because no abrasive forces are required for stirring.
Keywords
Animals, Chickens, Glass, *Kinetics, L-Lactate Dehydrogenase/*metabolism, Methods, Myocardium/enzymology, Solubility, Spectrophotometry, Ultraviolet, Temperature, Time Factors, Trypsin/*metabolism
Pubmed
Web of science
Create date
13/08/2015 8:53
Last modification date
20/08/2019 15:06
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