Article: article from journal or magazin.
Trimeric G protein-dependent frizzled signaling in Drosophila.
Frizzled (Fz) proteins are serpentine receptors that transduce critical cellular signals during development. Serpentine receptors usually signal to downstream effectors through an associated trimeric G protein complex. However, clear evidence for the role of trimeric G protein complexes for the Fz family of receptors has hitherto been lacking. Here, we show roles for the Galpha(o) subunit (Go) in mediating the two distinct pathways transduced by Fz receptors in Drosophila: the Wnt and planar polarity pathways. Go is required for transduction of both pathways, and epistasis experiments suggest that it is an immediate transducer of Fz. While overexpression effects of the wild-type form are receptor dependent, the activated form (Go-GTP) can signal when the receptor is removed. Thus, Go is likely part of a trimeric G protein complex that directly transduces Fz signals from the membrane to downstream components.
Animals, Cell Polarity/genetics, Cell Polarity/physiology, Drosophila, Drosophila Proteins/metabolism, Frizzled Receptors, GTP-Binding Protein alpha Subunits, Gi-Go/genetics, GTP-Binding Protein alpha Subunits, Gi-Go/physiology, Green Fluorescent Proteins/genetics, Intercellular Signaling Peptides and Proteins/metabolism, Membrane Proteins/metabolism, Phenotype, Proto-Oncogene Proteins/metabolism, Receptors, G-Protein-Coupled, Signal Transduction, Wing/growth & development, Wing/metabolism, Wnt Proteins, Wnt1 Protein
Web of science
Last modification date