Article: article from journal or magazin.
A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.
Applied and Environmental Microbiology
By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter.
Bacillus subtilis/genetics, Bacillus subtilis/physiology, Bacterial Proteins/genetics, DNA-Binding Proteins/genetics, Flow Cytometry, Gene Expression Regulation, Bacterial, Isopropyl Thiogalactoside/pharmacology, Promoter Regions, Genetic, Spores, Bacterial/physiology, Toxoids/genetics, Transcription Factors/genetics
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