A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.
Details
Serval ID
serval:BIB_48C948B89901
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.
Journal
Applied and Environmental Microbiology
ISSN
0099-2240 (Print)
ISSN-L
0099-2240
Publication state
Published
Issued date
2007
Volume
73
Number
7
Pages
2390-2393
Language
english
Abstract
By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter.
Keywords
Bacillus subtilis/genetics, Bacillus subtilis/physiology, Bacterial Proteins/genetics, DNA-Binding Proteins/genetics, Flow Cytometry, Gene Expression Regulation, Bacterial, Isopropyl Thiogalactoside/pharmacology, Promoter Regions, Genetic, Spores, Bacterial/physiology, Toxoids/genetics, Transcription Factors/genetics
Pubmed
Web of science
Open Access
Yes
Create date
11/10/2016 16:26
Last modification date
20/08/2019 14:55
Usage data
