A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.
Détails
ID Serval
serval:BIB_48C948B89901
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A derepression system based on the Bacillus subtilis sporulation pathway offers dynamic control of heterologous gene expression.
Périodique
Applied and Environmental Microbiology
ISSN
0099-2240 (Print)
ISSN-L
0099-2240
Statut éditorial
Publié
Date de publication
2007
Volume
73
Numéro
7
Pages
2390-2393
Langue
anglais
Résumé
By rewiring the sporulation gene-regulatory network of Bacillus subtilis, we generated a novel expression system relying on derepression. The gene of interest is placed under the control of the abrB promoter, which is active only when Spo0A is absent, and Spo0A is controlled via an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter.
Mots-clé
Bacillus subtilis/genetics, Bacillus subtilis/physiology, Bacterial Proteins/genetics, DNA-Binding Proteins/genetics, Flow Cytometry, Gene Expression Regulation, Bacterial, Isopropyl Thiogalactoside/pharmacology, Promoter Regions, Genetic, Spores, Bacterial/physiology, Toxoids/genetics, Transcription Factors/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/10/2016 16:26
Dernière modification de la notice
20/08/2019 14:55
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