ERK signaling pathway regulates sleep duration through activity-induced gene expression during wakefulness.

Détails

Ressource 1Télécharger: 5_28119463_Postprint.pdf (1396.45 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_3F8B9D2AAC2B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
ERK signaling pathway regulates sleep duration through activity-induced gene expression during wakefulness.
Périodique
Science signaling
Auteur(s)
Mikhail C., Vaucher A., Jimenez S., Tafti M.
ISSN
1937-9145 (Electronic)
ISSN-L
1945-0877
Statut éditorial
Publié
Date de publication
24/01/2017
Peer-reviewed
Oui
Volume
10
Numéro
463
Pages
463
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Wakefulness is accompanied by experience-dependent synaptic plasticity and an increase in activity-regulated gene transcription. Wake-induced genes are certainly markers of neuronal activity and may also directly regulate the duration of and need for sleep. We stimulated murine cortical cultures with the neuromodulatory signals that are known to control wakefulness in the brain and found that norepinephrine alone or a mixture of these neuromodulators induced activity-regulated gene transcription. Pharmacological inhibition of the various signaling pathways involved in the regulation of gene expression indicated that the extracellular signal-regulated kinase (ERK) pathway is the principal one mediating the effects of waking neuromodulators on gene expression. In mice, ERK phosphorylation in the cortex increased and decreased with wakefulness and sleep. Whole-body or cortical neuron-specific deletion of Erk1 or Erk2 significantly increased the duration of wakefulness in mice, and pharmacological inhibition of ERK phosphorylation decreased sleep duration and increased the duration of wakefulness bouts. Thus, this signaling pathway, which is highly conserved from Drosophila to mammals, is a key pathway that links waking experience-induced neuronal gene expression to sleep duration and quality.

Mots-clé
Animals, Blotting, Western, Cells, Cultured, Cytoskeletal Proteins/genetics, Cytoskeletal Proteins/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Gene Expression/drug effects, Gene Expression/genetics, Homer Scaffolding Proteins/genetics, Homer Scaffolding Proteins/metabolism, MAP Kinase Signaling System/drug effects, MAP Kinase Signaling System/genetics, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Mitogen-Activated Protein Kinase 1/genetics, Mitogen-Activated Protein Kinase 1/metabolism, Mitogen-Activated Protein Kinase 3/genetics, Mitogen-Activated Protein Kinase 3/metabolism, Nerve Tissue Proteins/genetics, Nerve Tissue Proteins/metabolism, Neurotransmitter Agents/pharmacology, Phosphorylation, Reverse Transcriptase Polymerase Chain Reaction, Sleep/genetics, Sleep Deprivation, Time Factors, Transcription Factors/genetics, Transcription Factors/metabolism, Wakefulness/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
30/01/2017 19:32
Dernière modification de la notice
08/05/2019 17:33
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