Onychomycosis insensitive to systemic terbinafine and azole treatments reveals non-dermatophyte moulds as infectious agents.

Details

Serval ID
serval:BIB_382F35C99528
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Institution
Title
Onychomycosis insensitive to systemic terbinafine and azole treatments reveals non-dermatophyte moulds as infectious agents.
Journal
Dermatology
Author(s)
Baudraz-Rosselet Florence, Ruffieux Catherine, Lurati Massimo, Bontems Olympia, Monod Michel
ISSN
1421-9832[electronic], 1018-8665[linking]
Publication state
Published
Issued date
2010
Volume
220
Number
2
Pages
164-168
Language
english
Abstract
BACKGROUND: Dermatophytes are the main cause of onychomycoses, but various non-dermatophyte filamentous fungi are often isolated from abnormal nails. OBJECTIVE: Our aim was the in situ identification of the fungal infectious agent in 8 cases of onychomycoses which could not be cured after systemic terbinafine and itraconazole treatment. METHODS: Fungal DNA was extracted from nail samples, and infectious fungi were identified by restriction fragment length polymorphism (RFLP) of amplified fungal ribosomal DNA using a previously described PCR/RFLP assay. RESULTS: PCR/RFLP identification of fungi in nails allows the identification of the infectious agent: Fusarium sp., Acremonium sp. and Aspergillus sp. were found as a sole infectious agent in 5, 2 and 1 cases, respectively. CONCLUSIONS: Fusarium spp. and other non-dermatophyte filamentous fungi are especially difficult to cure in onychomycoses utilising standard treatment with terbinafine and itraconazole. PCR fungal identification helps demonstrate the presence of moulds in order to prescribe alternative antifungal treatments.
Keywords
Trichophyton, Fusarium, Acremonium, Aspergillus, Onychomycosis, Fungi, Identification, Assay, Nail
Pubmed
Web of science
Create date
29/03/2010 15:19
Last modification date
20/08/2019 13:26
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