Dexamethasone treatment increases neuropeptide Y levels in rat hypothalamic neurones
Details
Serval ID
serval:BIB_32A7AE507CD6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dexamethasone treatment increases neuropeptide Y levels in rat hypothalamic neurones
Journal
Life Sciences
ISSN
0024-3205 (Print)
Publication state
Published
Issued date
1988
Volume
43
Number
23
Pages
1879-86
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, Non-U.S. Gov't
Abstract
Immunoreactive glucocorticoid receptors (GR) have previously been demonstrated in neuropeptide Y (NPY) neurones of the rat hypothalamus. To determine whether NPY synthesis is influenced by glucocorticoids, the effect of dexamethasone (DEX) on the levels of immunoreactive NPY in rat hypothalamic neurones was investigated in vivo and in vitro. Daily injections of DEX (0.1 mg/day) for 5 days increased the NPY content of the mediobasal hypothalamus in female rats by 117% (p less than 0.002). Primary cultures of hypothalamic neurones were also sensitive to the effect of glucocorticoids. Intracellular NPY levels were significantly increased (p less than 0.001) compared to control values by 151%, 222% and 268% when cultures were maintained in a defined serum free medium containing DEX 10(-9), 10(-8) and 10(-7) M respectively.
Keywords
Animals
Cells, Cultured
Dexamethasone/*pharmacology
Dose-Response Relationship, Drug
Female
Hypothalamus/drug effects/*metabolism
Median Eminence/drug effects/metabolism
Neurons/drug effects/*metabolism
Neuropeptide Y/*metabolism
Rats
Rats, Inbred Strains
Pubmed
Web of science
Create date
25/01/2008 17:26
Last modification date
20/08/2019 14:18