The glycoinositol-phospholipids of Phytomonas.
Details
Serval ID
serval:BIB_31476BAE97AC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The glycoinositol-phospholipids of Phytomonas.
Journal
Biochemical Journal
ISSN
0264-6021 (Print)
ISSN-L
0264-6021
Publication state
Published
Issued date
1995
Volume
311
Number
2
Pages
495-503
Language
english
Abstract
The Phytomonas spp. are trypanosomatid parasites of plants. A polar glycolipid fraction of a Phytomonas sp., isolated from the plant Euphorbia characias and grown in culture, was fractionated into four major glycolipid species (Phy 1-4). The glycolipids were analysed by chemical and enzymic modifications, composition and methylation analyses, electrospray mass spectrometry and microsequencing after HNO2 deamination and NaB3H4 reduction. The water-soluble headgroup of the Phy2 glycolipid was also analysed by 1H NMR. All four glycolipids were shown to be glycoinositol-phospholipids (GIPLs) with phosphatidylinositol (PI) moieties containing the fully saturated alkylacylglycerol lipids 1-O-hexadecyl-2-O-palmitoylglycerol and 1-O-hexadecyl-2-O-stearoylglycerol. The structures of the Phy 1-4 GIPLs are: Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6PI, Glc alpha 1-2(NH2-CH2CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6PI, [formula: see text] Glc alpha 1-2(NH2CH2CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2-CH2CH2-HPO4-)GlcN alpha 1-6PI [formula: see text] and Glc alpha 1-2Glc alpha 1-2(NH2CH2-CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2CH2CH2-HPO4-)-GlcN alpha 1-6PI. [formula: see text] The Phytomonas GIPLs represent a novel series of structures. This is the first description of the chemical structure of cell-surface molecules of this plant pathogen. The Phytomonas GIPLs are compared with those of other trypanosomatid parasites and are discussed with respect to trypanosomatid phylogenetic relationships.
Keywords
Animals, Carbohydrate Sequence, Chromatography, Thin Layer, Glycolipids/analysis, Glycolipids/chemistry, Glycosylphosphatidylinositols/analysis, Glycosylphosphatidylinositols/chemistry, Mass Spectrometry, Methylation, Molecular Sequence Data, Phosphatidylinositols/analysis, Plants/parasitology, Polysaccharides/analysis, Trypanosomatina/chemistry, Trypanosomatina/isolation &, purification
Pubmed
Web of science
Create date
19/01/2008 17:30
Last modification date
20/08/2019 13:16