The glycoinositol-phospholipids of Phytomonas.

Détails

ID Serval
serval:BIB_31476BAE97AC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The glycoinositol-phospholipids of Phytomonas.
Périodique
Biochemical Journal
Auteur⸱e⸱s
Redman C.A., Schneider P., Mehlert A., Ferguson M.A.
ISSN
0264-6021 (Print)
ISSN-L
0264-6021
Statut éditorial
Publié
Date de publication
1995
Volume
311
Numéro
2
Pages
495-503
Langue
anglais
Résumé
The Phytomonas spp. are trypanosomatid parasites of plants. A polar glycolipid fraction of a Phytomonas sp., isolated from the plant Euphorbia characias and grown in culture, was fractionated into four major glycolipid species (Phy 1-4). The glycolipids were analysed by chemical and enzymic modifications, composition and methylation analyses, electrospray mass spectrometry and microsequencing after HNO2 deamination and NaB3H4 reduction. The water-soluble headgroup of the Phy2 glycolipid was also analysed by 1H NMR. All four glycolipids were shown to be glycoinositol-phospholipids (GIPLs) with phosphatidylinositol (PI) moieties containing the fully saturated alkylacylglycerol lipids 1-O-hexadecyl-2-O-palmitoylglycerol and 1-O-hexadecyl-2-O-stearoylglycerol. The structures of the Phy 1-4 GIPLs are: Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6PI, Glc alpha 1-2(NH2-CH2CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN alpha 1-6PI, [formula: see text] Glc alpha 1-2(NH2CH2CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2-CH2CH2-HPO4-)GlcN alpha 1-6PI [formula: see text] and Glc alpha 1-2Glc alpha 1-2(NH2CH2-CH2-HPO4-)Man alpha 1-2Man alpha 1-6Man alpha 1-4(NH2CH2CH2-HPO4-)-GlcN alpha 1-6PI. [formula: see text] The Phytomonas GIPLs represent a novel series of structures. This is the first description of the chemical structure of cell-surface molecules of this plant pathogen. The Phytomonas GIPLs are compared with those of other trypanosomatid parasites and are discussed with respect to trypanosomatid phylogenetic relationships.
Mots-clé
Animals, Carbohydrate Sequence, Chromatography, Thin Layer, Glycolipids/analysis, Glycolipids/chemistry, Glycosylphosphatidylinositols/analysis, Glycosylphosphatidylinositols/chemistry, Mass Spectrometry, Methylation, Molecular Sequence Data, Phosphatidylinositols/analysis, Plants/parasitology, Polysaccharides/analysis, Trypanosomatina/chemistry, Trypanosomatina/isolation &amp, purification
Pubmed
Web of science
Création de la notice
19/01/2008 17:30
Dernière modification de la notice
20/08/2019 13:16
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