Time-Lapse Imaging of Glial-Axonal Interactions.

Details

Serval ID
serval:BIB_2E12C42F30EF
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Time-Lapse Imaging of Glial-Axonal Interactions.
Journal
Current protocols in neuroscience
Author(s)
Ioannidou K., Edgar J.M., Barnett S.C.
ISSN
1934-8576 (Electronic)
ISSN-L
1934-8576
Publication state
Published
Issued date
01/07/2015
Peer-reviewed
Oui
Volume
72
Pages
2.23.1-14
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
In the central nervous system (CNS), myelin is formed by oligodendrocytes that are derived from precursor cells, known as oligodendrocyte precursor cells (OPCs). Successive stages of OPC interactions with the axons can be visualized in vitro and ex vivo using mixed neural cell cultures and pieces of intact spinal cord, respectively. OPCs and their differentiation can be imaged using cell-type-specific markers or green fluorescent protein (GFP) tags. This protocol describes methodology for generating these two systems for time-lapse imaging of dynamic cell interactions using fluorescent and 2-photon microscopy.

Keywords
Animals, Axons/physiology, Central Nervous System/cytology, Fluorescent Dyes/metabolism, Neuroglia/cytology, Neuroglia/microbiology, Time-Lapse Imaging/methods
Pubmed
Create date
11/10/2016 15:29
Last modification date
20/08/2019 13:12
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