Time-Lapse Imaging of Glial-Axonal Interactions.

Détails

ID Serval
serval:BIB_2E12C42F30EF
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Time-Lapse Imaging of Glial-Axonal Interactions.
Périodique
Current protocols in neuroscience
Auteur⸱e⸱s
Ioannidou K., Edgar J.M., Barnett S.C.
ISSN
1934-8576 (Electronic)
ISSN-L
1934-8576
Statut éditorial
Publié
Date de publication
01/07/2015
Peer-reviewed
Oui
Volume
72
Pages
2.23.1-14
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
In the central nervous system (CNS), myelin is formed by oligodendrocytes that are derived from precursor cells, known as oligodendrocyte precursor cells (OPCs). Successive stages of OPC interactions with the axons can be visualized in vitro and ex vivo using mixed neural cell cultures and pieces of intact spinal cord, respectively. OPCs and their differentiation can be imaged using cell-type-specific markers or green fluorescent protein (GFP) tags. This protocol describes methodology for generating these two systems for time-lapse imaging of dynamic cell interactions using fluorescent and 2-photon microscopy.

Mots-clé
Animals, Axons/physiology, Central Nervous System/cytology, Fluorescent Dyes/metabolism, Neuroglia/cytology, Neuroglia/microbiology, Time-Lapse Imaging/methods
Pubmed
Création de la notice
11/10/2016 15:29
Dernière modification de la notice
20/08/2019 13:12
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