Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension

Details

Serval ID
serval:BIB_268F6569D44F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension
Journal
Nature Biotechnology
Author(s)
Schense  J. C., Bloch  J., Aebischer  P., Hubbell  J. A.
ISSN
1087-0156
Publication state
Published
Issued date
04/2000
Peer-reviewed
Oui
Volume
18
Number
4
Pages
415-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
Fibrin plays an important role in wound healing and regeneration, and enjoys widespread use in surgery and tissue engineering. The enzymatic activity of Factor XIIIa was employed to covalently incorporate exogenous bioactive peptides within fibrin during coagulation. Fibrin gels were formed with incorporated peptides from laminin and N-cadherin alone and in combination at concentrations up to 8.2 mol peptide per mole of fibrinogen. Neurite extension in vitro was enhanced when gels were augmented with exogenous peptide, with the maximal improvement reaching 75%. When this particular fibrin derivative was evaluated in rats in the repair of the severed dorsal root within polymeric tubes, the number of regenerated axons was enhanced by 85% relative to animals treated with tubes filled with unmodified fibrin. These results demonstrate that it is possible to enhance the biological activity of fibrin by enzymatically incorporating exogenous oligopeptide domains of morphoregulatory proteins.
Keywords
Amino Acid Sequence Animals *Cadherins/pharmacology Chick Embryo *Fibrin Fibrinogen Ganglia, Spinal/cytology/physiology Gels *Laminin/pharmacology Male Nerve Regeneration/drug effects/*physiology Neurites/drug effects/*physiology/ultrastructure Neurons/cytology/drug effects/*physiology *Peptide Fragments/pharmacology Rats Rats, Wistar Spinal Nerve Roots/cytology/physiology Transglutaminases/metabolism
Pubmed
Web of science
Create date
06/02/2008 10:03
Last modification date
20/08/2019 13:05
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