Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension
Détails
ID Serval
serval:BIB_268F6569D44F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension
Périodique
Nature Biotechnology
ISSN
1087-0156
Statut éditorial
Publié
Date de publication
04/2000
Peer-reviewed
Oui
Volume
18
Numéro
4
Pages
415-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
Fibrin plays an important role in wound healing and regeneration, and enjoys widespread use in surgery and tissue engineering. The enzymatic activity of Factor XIIIa was employed to covalently incorporate exogenous bioactive peptides within fibrin during coagulation. Fibrin gels were formed with incorporated peptides from laminin and N-cadherin alone and in combination at concentrations up to 8.2 mol peptide per mole of fibrinogen. Neurite extension in vitro was enhanced when gels were augmented with exogenous peptide, with the maximal improvement reaching 75%. When this particular fibrin derivative was evaluated in rats in the repair of the severed dorsal root within polymeric tubes, the number of regenerated axons was enhanced by 85% relative to animals treated with tubes filled with unmodified fibrin. These results demonstrate that it is possible to enhance the biological activity of fibrin by enzymatically incorporating exogenous oligopeptide domains of morphoregulatory proteins.
Mots-clé
Amino Acid Sequence
Animals
*Cadherins/pharmacology
Chick Embryo
*Fibrin
Fibrinogen
Ganglia, Spinal/cytology/physiology
Gels
*Laminin/pharmacology
Male
Nerve Regeneration/drug effects/*physiology
Neurites/drug effects/*physiology/ultrastructure
Neurons/cytology/drug effects/*physiology
*Peptide Fragments/pharmacology
Rats
Rats, Wistar
Spinal Nerve Roots/cytology/physiology
Transglutaminases/metabolism
Pubmed
Web of science
Création de la notice
06/02/2008 11:03
Dernière modification de la notice
20/08/2019 14:05