Article: article from journal or magazin.
Dietary fish oil is antihypertrophic but does not enhance postischemic myocardial function in female mice.
American journal of physiology. Heart and circulatory physiology
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Clinically and experimentally, a case for omega-3 polyunsaturated fatty acid (PUFA) cardioprotection in females has not been clearly established. The goal of this study was to investigate whether dietary omega-3 PUFA supplementation could provide ischemic protection in female mice with an underlying genetic predisposition to cardiac hypertrophy. Mature female transgenic mice (TG) with cardiac-specific overexpression of angiotensinogen that develop normotensive cardiac hypertrophy and littermate wild-type (WT) mice were fed a fish oil-derived diet (FO) or PUFA-matched control diet (CTR) for 4 wk. Myocardial membrane lipids, ex vivo cardiac performance (intraventricular balloon) after global no-flow ischemia and reperfusion (15/30 min), and reperfusion arrhythmia incidence were assessed. FO diet suppressed cardiac growth by 5% and 10% in WT and TG, respectively (P < 0.001). The extent of mechanical recovery [rate-pressure product (RPP) = beats/min x mmHg] of FO-fed WT and TG hearts was similar (50 +/- 7% vs. 45 +/- 12%, 30 min reperfusion), and this was not significantly different from CTR-fed WT or TG. To evaluate whether systemic estrogen was masking a protective effect of the FO diet, the responses of ovariectomized (OVX) WT and TG mice to FO dietary intervention were assessed. The extent of mechanical recovery of FO-fed OVX WT and TG (RPP, 50 +/- 4% vs. 64 +/- 8%) was not enhanced compared with CTR-fed mice (RPP, 60 +/- 11% vs. 80 +/- 8%, P = 0.335). Dietary FO did not suppress the incidence of reperfusion arrhythmias in WT or TG hearts (ovary-intact mice or OVX). Our findings indicate a lack of cardioprotective effect of dietary FO in females, determined by assessment of mechanical and arrhythmic activity postischemia in a murine ex vivo heart model.
Angiotensin II/genetics, Angiotensin II/metabolism, Angiotensinogen/genetics, Angiotensinogen/metabolism, Animals, Arrhythmias, Cardiac/etiology, Dietary Fats, Unsaturated/pharmacology, Disease Models, Animal, Estrogens/metabolism, Female, Fish Oils/pharmacology, Genetic Predisposition to Disease/genetics, Hypertrophy/genetics, Hypertrophy/prevention & control, Mice, Mice, Transgenic, Myocardial Ischemia/complications, Myocardial Ischemia/physiopathology, Myocardium/metabolism, Myocardium/pathology, Ovariectomy, Reperfusion Injury/complications, Reperfusion Injury/physiopathology
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