A global genetic interaction network maps a wiring diagram of cellular function.

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Serval ID
serval:BIB_1A8881DA9334
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Production externe
Title
A global genetic interaction network maps a wiring diagram of cellular function.
Journal
Science
Author(s)
Costanzo M., VanderSluis B., Koch E.N., Baryshnikova A., Pons C., Tan G., Wang W., Usaj M., Hanchard J., Lee S.D., Pelechano V., Styles E.B., Billmann M., van Leeuwen J., van Dyk N., Lin Z.Y., Kuzmin E., Nelson J., Piotrowski J.S., Srikumar T., Bahr S., Chen Y., Deshpande R., Kurat C.F., Li S.C., Li Z., Usaj M.M., Okada H., Pascoe N., San Luis B.J., Sharifpoor S., Shuteriqi E., Simpkins S.W., Snider J., Suresh H.G., Tan Y., Zhu H., Malod-Dognin N., Janjic V., Przulj N., Troyanskaya O.G., Stagljar I., Xia T., Ohya Y., Gingras A.C., Raught B., Boutros M., Steinmetz L.M., Moore C.L., Rosebrock A.P., Caudy A.A., Myers C.L., Andrews B., Boone C.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Publication state
Published
Issued date
23/09/2016
Peer-reviewed
Oui
Volume
353
Number
6306
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Abstract
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to protein complexes and pathways, biological processes, and cellular compartments. Negative interactions connected functionally related genes, mapped core bioprocesses, and identified pleiotropic genes, whereas positive interactions often mapped general regulatory connections among gene pairs, rather than shared functionality. The global network illustrates how coherent sets of genetic interactions connect protein complex and pathway modules to map a functional wiring diagram of the cell.
Keywords
Epistasis, Genetic, Gene Regulatory Networks, Genes, Essential, Genes, Fungal/physiology, Genetic Pleiotropy/physiology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics
DOI
10.1126/science.aaf1420
Pubmed
27708008
Web of science
000383708700030
Open Access
Yes
Create date
22/01/2019 16:01
Last modification date
21/08/2019 5:35
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