A global genetic interaction network maps a wiring diagram of cellular function.

Détails

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ID Serval
serval:BIB_1A8881DA9334
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Production externe
Titre
A global genetic interaction network maps a wiring diagram of cellular function.
Périodique
Science
Auteur⸱e⸱s
Costanzo M., VanderSluis B., Koch E.N., Baryshnikova A., Pons C., Tan G., Wang W., Usaj M., Hanchard J., Lee S.D., Pelechano V., Styles E.B., Billmann M., van Leeuwen J., van Dyk N., Lin Z.Y., Kuzmin E., Nelson J., Piotrowski J.S., Srikumar T., Bahr S., Chen Y., Deshpande R., Kurat C.F., Li S.C., Li Z., Usaj M.M., Okada H., Pascoe N., San Luis B.J., Sharifpoor S., Shuteriqi E., Simpkins S.W., Snider J., Suresh H.G., Tan Y., Zhu H., Malod-Dognin N., Janjic V., Przulj N., Troyanskaya O.G., Stagljar I., Xia T., Ohya Y., Gingras A.C., Raught B., Boutros M., Steinmetz L.M., Moore C.L., Rosebrock A.P., Caudy A.A., Myers C.L., Andrews B., Boone C.
ISSN
1095-9203 (Electronic)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
23/09/2016
Peer-reviewed
Oui
Volume
353
Numéro
6306
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural
Publication Status: ppublish
Résumé
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. Genetic interaction profiles enabled assembly of a hierarchical model of cell function, including modules corresponding to protein complexes and pathways, biological processes, and cellular compartments. Negative interactions connected functionally related genes, mapped core bioprocesses, and identified pleiotropic genes, whereas positive interactions often mapped general regulatory connections among gene pairs, rather than shared functionality. The global network illustrates how coherent sets of genetic interactions connect protein complex and pathway modules to map a functional wiring diagram of the cell.
Mots-clé
Epistasis, Genetic, Gene Regulatory Networks, Genes, Essential, Genes, Fungal/physiology, Genetic Pleiotropy/physiology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics
DOI
10.1126/science.aaf1420
Pubmed
27708008
Web of science
000383708700030
Open Access
Oui
Création de la notice
22/01/2019 17:01
Dernière modification de la notice
21/08/2019 6:35
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