Sensitive identification of neoantigens and cognate TCRs in human solid tumors.
Details
Serval ID
serval:BIB_18BFBDDF727B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sensitive identification of neoantigens and cognate TCRs in human solid tumors.
Journal
Nature biotechnology
ISSN
1546-1696 (Electronic)
ISSN-L
1087-0156
Publication state
Published
Issued date
05/2022
Peer-reviewed
Oui
Volume
40
Number
5
Pages
656-660
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
The identification of patient-specific tumor antigens is complicated by the low frequency of T cells specific for each tumor antigen. Here we describe NeoScreen, a method that enables the sensitive identification of rare tumor (neo)antigens and of cognate T cell receptors (TCRs) expressed by tumor-infiltrating lymphocytes. T cells transduced with tumor antigen-specific TCRs identified by NeoScreen mediate regression of established tumors in patient-derived xenograft mice.
Keywords
Animals, Antigens, Neoplasm/genetics, CD8-Positive T-Lymphocytes, Humans, Lymphocytes, Tumor-Infiltrating, Mice, Neoplasms/genetics, Neoplasms/therapy, Receptors, Antigen, T-Cell/genetics, T-Lymphocytes
Pubmed
Web of science
Open Access
Yes
Create date
26/11/2021 18:08
Last modification date
23/11/2022 7:08