Ultrasensitive reporter protein detection in genetically engineered bacteria.

Details

Serval ID
serval:BIB_1771FB324C30
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ultrasensitive reporter protein detection in genetically engineered bacteria.
Journal
Analytical Chemistry
Author(s)
Wells M., Gösch M., Rigler R., Harms H., Lasser T., van der Meer J.R.
ISSN
0003-2700[print], 0003-2700[linking]
Publication state
Published
Issued date
2005
Volume
77
Number
9
Pages
2683-2689
Language
english
Abstract
We demonstrate the use of laser-induced fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates and, since the spectroscopic focal volume is approximately the size of one bioreporter cell, also in individual live bacteria. This is, to our knowledge, the first ever proof-of-concept work utilizing instrumentation with single-molecule detection capability to monitor bioreporter response. Although we use arsenic inducible bioreporters here, the method is extensible to gfp/egfp bioreporters that are responsive to other substances.
Keywords
Arsenites/analysis, Escherichia coli/chemistry, Escherichia coli/genetics, Green Fluorescent Proteins/analysis, Green Fluorescent Proteins/biosynthesis, Organisms, Genetically Modified/metabolism, Recombinant Proteins/analysis, Recombinant Proteins/biosynthesis, Spectrometry, Fluorescence/methods
Pubmed
Web of science
Create date
21/01/2008 13:35
Last modification date
20/08/2019 12:47
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