Ultrasensitive reporter protein detection in genetically engineered bacteria.

Détails

ID Serval
serval:BIB_1771FB324C30
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Ultrasensitive reporter protein detection in genetically engineered bacteria.
Périodique
Analytical Chemistry
Auteur⸱e⸱s
Wells M., Gösch M., Rigler R., Harms H., Lasser T., van der Meer J.R.
ISSN
0003-2700[print], 0003-2700[linking]
Statut éditorial
Publié
Date de publication
2005
Volume
77
Numéro
9
Pages
2683-2689
Langue
anglais
Résumé
We demonstrate the use of laser-induced fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates and, since the spectroscopic focal volume is approximately the size of one bioreporter cell, also in individual live bacteria. This is, to our knowledge, the first ever proof-of-concept work utilizing instrumentation with single-molecule detection capability to monitor bioreporter response. Although we use arsenic inducible bioreporters here, the method is extensible to gfp/egfp bioreporters that are responsive to other substances.
Mots-clé
Arsenites/analysis, Escherichia coli/chemistry, Escherichia coli/genetics, Green Fluorescent Proteins/analysis, Green Fluorescent Proteins/biosynthesis, Organisms, Genetically Modified/metabolism, Recombinant Proteins/analysis, Recombinant Proteins/biosynthesis, Spectrometry, Fluorescence/methods
Pubmed
Web of science
Création de la notice
21/01/2008 14:35
Dernière modification de la notice
20/08/2019 13:47
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