Highly inducible synthesis of heterologous proteins in epithelial cells carrying a glucocorticoid-responsive vector.
Details
Serval ID
serval:BIB_170BE82CCAAF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Highly inducible synthesis of heterologous proteins in epithelial cells carrying a glucocorticoid-responsive vector.
Journal
Gene
ISSN
0378-1119 (Print)
ISSN-L
0378-1119
Publication state
Published
Issued date
1992
Volume
111
Number
2
Pages
199-206
Language
english
Abstract
A glucocorticoid-responsive vector is described which allows for the highly inducible expression of complementary DNAs (cDNAs) in stably transfected mammalian cell lines. This vector, pLK-neo, composed of a variant mouse mammary tumor virus long terminal repeat promoter, containing a hormone regulatory element, a Geneticin resistance-encoding gene in a simian virus 40 transcription unit, and a polylinker insertion site for heterologous cDNAs, was used to express the polymeric immunoglobulin (poly-Ig) receptor and the thymocyte marker, Thy-1, in Madin-Darby canine kidney (MDCK) cells and in murine fibroblast L cells. A high level of poly-Ig receptor or Thy-1 mRNA accumulation was observed in MDCK cells in response to dexamethasone with a parallel ten- to 200-fold increase in protein synthesis depending on the recombinant protein and the transfected cell clone.
Keywords
Animals, Antigens, Surface/genetics, Antigens, Thy-1, Cell Line, Dexamethasone/pharmacology, Gene Expression Regulation/drug effects, Genetic Vectors/genetics, Mammary Tumor Virus, Mouse/genetics, Membrane Glycoproteins/genetics, Mice, Plasmids/genetics, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Repetitive Sequences, Nucleic Acid/genetics, Secretory Component/genetics, Transfection/genetics
Pubmed
Web of science
Create date
24/01/2008 15:02
Last modification date
20/08/2019 12:46
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