Article: article from journal or magazin.
Highly inducible synthesis of heterologous proteins in epithelial cells carrying a glucocorticoid-responsive vector.
A glucocorticoid-responsive vector is described which allows for the highly inducible expression of complementary DNAs (cDNAs) in stably transfected mammalian cell lines. This vector, pLK-neo, composed of a variant mouse mammary tumor virus long terminal repeat promoter, containing a hormone regulatory element, a Geneticin resistance-encoding gene in a simian virus 40 transcription unit, and a polylinker insertion site for heterologous cDNAs, was used to express the polymeric immunoglobulin (poly-Ig) receptor and the thymocyte marker, Thy-1, in Madin-Darby canine kidney (MDCK) cells and in murine fibroblast L cells. A high level of poly-Ig receptor or Thy-1 mRNA accumulation was observed in MDCK cells in response to dexamethasone with a parallel ten- to 200-fold increase in protein synthesis depending on the recombinant protein and the transfected cell clone.
Animals, Antigens, Surface/genetics, Antigens, Thy-1, Cell Line, Dexamethasone/pharmacology, Gene Expression Regulation/drug effects, Genetic Vectors/genetics, Mammary Tumor Virus, Mouse/genetics, Membrane Glycoproteins/genetics, Mice, Plasmids/genetics, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Repetitive Sequences, Nucleic Acid/genetics, Secretory Component/genetics, Transfection/genetics
Web of science
Last modification date