Highly inducible synthesis of heterologous proteins in epithelial cells carrying a glucocorticoid-responsive vector.

Détails

ID Serval
serval:BIB_170BE82CCAAF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Highly inducible synthesis of heterologous proteins in epithelial cells carrying a glucocorticoid-responsive vector.
Périodique
Gene
Auteur⸱e⸱s
Hirt R.P., Poulain-Godefroy O., Billotte J., Kraehenbuhl J.P., Fasel N.
ISSN
0378-1119 (Print)
ISSN-L
0378-1119
Statut éditorial
Publié
Date de publication
1992
Volume
111
Numéro
2
Pages
199-206
Langue
anglais
Résumé
A glucocorticoid-responsive vector is described which allows for the highly inducible expression of complementary DNAs (cDNAs) in stably transfected mammalian cell lines. This vector, pLK-neo, composed of a variant mouse mammary tumor virus long terminal repeat promoter, containing a hormone regulatory element, a Geneticin resistance-encoding gene in a simian virus 40 transcription unit, and a polylinker insertion site for heterologous cDNAs, was used to express the polymeric immunoglobulin (poly-Ig) receptor and the thymocyte marker, Thy-1, in Madin-Darby canine kidney (MDCK) cells and in murine fibroblast L cells. A high level of poly-Ig receptor or Thy-1 mRNA accumulation was observed in MDCK cells in response to dexamethasone with a parallel ten- to 200-fold increase in protein synthesis depending on the recombinant protein and the transfected cell clone.
Mots-clé
Animals, Antigens, Surface/genetics, Antigens, Thy-1, Cell Line, Dexamethasone/pharmacology, Gene Expression Regulation/drug effects, Genetic Vectors/genetics, Mammary Tumor Virus, Mouse/genetics, Membrane Glycoproteins/genetics, Mice, Plasmids/genetics, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Repetitive Sequences, Nucleic Acid/genetics, Secretory Component/genetics, Transfection/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 15:02
Dernière modification de la notice
20/08/2019 12:46
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