Clonal deletion of self-reactive T cells in irradiation bone marrow chimeras and neonatally tolerant mice. Evidence for intercellular transfer of Mlsa
Details
Serval ID
serval:BIB_14E6C009C52F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Clonal deletion of self-reactive T cells in irradiation bone marrow chimeras and neonatally tolerant mice. Evidence for intercellular transfer of Mlsa
Journal
Journal of Experimental Medicine
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
08/1989
Volume
170
Number
2
Pages
595-600
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 1
Research Support, Non-U.S. Gov't --- Old month value: Aug 1
Abstract
Tolerance to Mlsa has been shown to be associated with clonal deletion of cells carrying TCR beta chain variable regions V beta 6 or V beta 8.1 in mice possessing I-E antigens. To evaluate the rules of tolerance induction to Mlsa we prepared irradiation bone marrow chimeras expressing Mlsa or Mlsb and I-E by different cell types. Deletion of V beta 6+, Mlsa-reactive T cells required the presence of Mlsa and I-E products either on bone marrow-derived cells or on irradiated recipient cells. Tolerance was induced when Mlsa and I-E were expressed by distinct cells of the chimera. Also neonatally tolerized mice exhibited depletion of V beta 6+ cells after injection of I-E- Mlsa spleen cells (DBA/1) into newborn I-E+ Mlsb mice (BALB/c x B10.G)F1. These results suggest that the product of the Mlsa locus is soluble and/or may be transferred from cell to cell and bound to I-E antigens. The chimera experiments also showed that tolerance to Mlsa is H-2 allele independent, i.e., is apparently unrestricted. Differentiation of chimeric (H-2d/Mlsa x H-2q/Mlsb)F1 stem cells in either an H-2d or an H-2q thymus revealed that tolerance assessed by absence of V beta 6+ T cells is not dependent on the thymically determined restriction specificity of T cells.
Keywords
Animals
Animals, Newborn/*immunology
Autoantigens/*immunology
Bone Marrow/*immunology
Bone Marrow Cells
Histocompatibility Antigens Class II/immunology
*Immune Tolerance
Mice
Mice, Inbred Strains
Radiation Chimera
Receptors, Antigen, T-Cell/immunology
Receptors, Antigen, T-Cell, alpha-beta
Spleen/cytology/immunology
T-Lymphocytes/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:32
Last modification date
20/08/2019 13:43