Clonal deletion of self-reactive T cells in irradiation bone marrow chimeras and neonatally tolerant mice. Evidence for intercellular transfer of Mlsa.

Détails

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Accès restreint UNIL
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_14E6C009C52F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Clonal deletion of self-reactive T cells in irradiation bone marrow chimeras and neonatally tolerant mice. Evidence for intercellular transfer of Mlsa.
Périodique
The Journal of experimental medicine
Auteur⸱e⸱s
Speiser D.E., Schneider R., Hengartner H., MacDonald H.R., Zinkernagel R.M.
ISSN
0022-1007
ISSN-L
0022-1007
Statut éditorial
Publié
Date de publication
01/08/1989
Peer-reviewed
Oui
Volume
170
Numéro
2
Pages
595-600
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Tolerance to Mlsa has been shown to be associated with clonal deletion of cells carrying TCR beta chain variable regions V beta 6 or V beta 8.1 in mice possessing I-E antigens. To evaluate the rules of tolerance induction to Mlsa we prepared irradiation bone marrow chimeras expressing Mlsa or Mlsb and I-E by different cell types. Deletion of V beta 6+, Mlsa-reactive T cells required the presence of Mlsa and I-E products either on bone marrow-derived cells or on irradiated recipient cells. Tolerance was induced when Mlsa and I-E were expressed by distinct cells of the chimera. Also neonatally tolerized mice exhibited depletion of V beta 6+ cells after injection of I-E- Mlsa spleen cells (DBA/1) into newborn I-E+ Mlsb mice (BALB/c x B10.G)F1. These results suggest that the product of the Mlsa locus is soluble and/or may be transferred from cell to cell and bound to I-E antigens. The chimera experiments also showed that tolerance to Mlsa is H-2 allele independent, i.e., is apparently unrestricted. Differentiation of chimeric (H-2d/Mlsa x H-2q/Mlsb)F1 stem cells in either an H-2d or an H-2q thymus revealed that tolerance assessed by absence of V beta 6+ T cells is not dependent on the thymically determined restriction specificity of T cells.
Mots-clé
Animals, Animals, Newborn/immunology, Autoantigens/immunology, Bone Marrow/immunology, Bone Marrow Cells, Histocompatibility Antigens Class II/immunology, Immune Tolerance, Mice, Mice, Inbred Strains, Radiation Chimera, Receptors, Antigen, T-Cell/immunology, Receptors, Antigen, T-Cell, alpha-beta, Spleen/cytology, Spleen/immunology, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:32
Dernière modification de la notice
09/08/2024 15:53
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