Article: article from journal or magazin.
IL-13 induces expression of CD36 in human monocytes through PPARgamma activation.
European Journal of Immunology
The class B scavenger receptor CD36 is a component of the pattern recognition receptors on monocytes that recognizes a variety of molecules. CD36 expression in monocytes depends on exposure to soluble mediators. We demonstrate here that CD36 expression is induced in human monocytes following exposure to IL-13, a Th2 cytokine, via the peroxisome proliferator-activated receptor (PPAR)gamma pathway. Induction of CD36 protein was paralleled by an increase in CD36 mRNA. The PPARgamma pathway was demonstrated using transfection of a PPARgamma expression plasmid into the murine macrophage cell line RAW264.7, expressing very low levels of PPARgamma, and in peritoneal macrophages from PPARgamma-conditional null mice. We also show that CD36 induction by IL-13 via PPARgamma is dependent on phospholipase A2 activation and that IL-13 induces the production of endogenous 15-deoxy-Delta12,14-prostaglandin J2, an endogenous PPARgamma ligand, and its nuclear localization in human monocytes. Finally, we demonstrate that CD36 and PPARgamma are involved in IL-13-mediated phagocytosis of Plasmodium falciparum-parasitized erythrocytes. These results reveal a novel role for PPARgamma in the alternative activation of monocytes by IL-13, suggesting that endogenous PPARgamma ligands, produced by phospholipase A2 activation, could contribute to the biochemical and cellular functions of CD36.
Anilides/pharmacology, Animals, Antigens, CD36/genetics, Antigens, CD36/metabolism, Arachidonic Acids/pharmacology, Cell Line, Tumor, DNA/metabolism, Enzyme Inhibitors/pharmacology, Erythrocytes/drug effects, Erythrocytes/parasitology, Gene Expression/drug effects, Humans, Hypoglycemic Agents/pharmacology, Interleukin-13/pharmacology, Macrophages/drug effects, Macrophages/metabolism, Macrophages, Peritoneal/drug effects, Macrophages, Peritoneal/metabolism, Mice, Monocytes/drug effects, Monocytes/metabolism, PPAR gamma/agonists, PPAR gamma/antagonists & inhibitors, Phagocytosis/drug effects, Phospholipases A/antagonists & inhibitors, Phospholipases A2, Phosphonic Acids/pharmacology, Plasmodium falciparum/physiology, Prostaglandin D2/analogs & derivatives, Prostaglandin D2/metabolism, Protein Binding/drug effects, Thiazolidinediones/pharmacology, Transfection
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