Article: article from journal or magazin.
Amplification of the housekeeping sigma factor in Pseudomonas fluorescens CHA0 enhances antibiotic production and improves biocontrol abilities.
Journal of Bacteriology
Pseudomonas fluorescens CHA0 produces a variety of secondary metabolites, in particular the antibiotics pyoluteorin and 2,4-diacetylphloroglucinol, and protects various plants from diseases caused by soilborne pathogenic fungi. The rpoD gene encoding the housekeeping sigma factor sigma 70 of P. fluorescens was sequenced. The deduced RpoD protein showed 83% identity with RpoD of Pseudomonas aeruginosa and 67% identity with RpoD of Escherichia coli. Attempts to inactivate the single chromosomal rpoD gene of strain CHA0 were unsuccessful, indicating an essential role of this gene. When rpoD was carried by an IncP vector in strain CHA0, the production of both antibiotics was increased severalfold and, in parallel, protection of cucumber against disease caused by Pythium ultimum was improved, in comparison with strain CHA0.
Amino Acid Sequence, Anti-Bacterial Agents/biosynthesis, Antifungal Agents/biosynthesis, Bacterial Proteins/biosynthesis, Bacterial Proteins/genetics, Base Sequence, Cucumis sativus/microbiology, DNA-Directed RNA Polymerases/biosynthesis, DNA-Directed RNA Polymerases/genetics, Gene Amplification/genetics, Genes, Bacterial/genetics, Molecular Sequence Data, Phenols, Phloroglucinol/analogs & derivatives, Phloroglucinol/metabolism, Plant Roots/microbiology, Pseudomonas fluorescens/genetics, Pseudomonas fluorescens/metabolism, Pyrroles, Pythium/physiology, Restriction Mapping, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sigma Factor/biosynthesis, Sigma Factor/genetics
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