Amplification of the housekeeping sigma factor in Pseudomonas fluorescens CHA0 enhances antibiotic production and improves biocontrol abilities.

Details

Serval ID
serval:BIB_124389F75F1C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Amplification of the housekeeping sigma factor in Pseudomonas fluorescens CHA0 enhances antibiotic production and improves biocontrol abilities.
Journal
Journal of Bacteriology
Author(s)
Schnider U., Keel C., Blumer C., Troxler J., Défago G., Haas D.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Publication state
Published
Issued date
1995
Volume
177
Number
18
Pages
5387-5392
Language
english
Abstract
Pseudomonas fluorescens CHA0 produces a variety of secondary metabolites, in particular the antibiotics pyoluteorin and 2,4-diacetylphloroglucinol, and protects various plants from diseases caused by soilborne pathogenic fungi. The rpoD gene encoding the housekeeping sigma factor sigma 70 of P. fluorescens was sequenced. The deduced RpoD protein showed 83% identity with RpoD of Pseudomonas aeruginosa and 67% identity with RpoD of Escherichia coli. Attempts to inactivate the single chromosomal rpoD gene of strain CHA0 were unsuccessful, indicating an essential role of this gene. When rpoD was carried by an IncP vector in strain CHA0, the production of both antibiotics was increased severalfold and, in parallel, protection of cucumber against disease caused by Pythium ultimum was improved, in comparison with strain CHA0.
Keywords
Amino Acid Sequence, Anti-Bacterial Agents/biosynthesis, Antifungal Agents/biosynthesis, Bacterial Proteins/biosynthesis, Bacterial Proteins/genetics, Base Sequence, Cucumis sativus/microbiology, DNA-Directed RNA Polymerases/biosynthesis, DNA-Directed RNA Polymerases/genetics, Gene Amplification/genetics, Genes, Bacterial/genetics, Molecular Sequence Data, Phenols, Phloroglucinol/analogs & derivatives, Phloroglucinol/metabolism, Plant Roots/microbiology, Pseudomonas fluorescens/genetics, Pseudomonas fluorescens/metabolism, Pyrroles, Pythium/physiology, Restriction Mapping, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sigma Factor/biosynthesis, Sigma Factor/genetics
Pubmed
Web of science
Create date
24/01/2008 14:51
Last modification date
20/08/2019 13:40
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