Amplification of the housekeeping sigma factor in Pseudomonas fluorescens CHA0 enhances antibiotic production and improves biocontrol abilities.

Détails

ID Serval
serval:BIB_124389F75F1C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Amplification of the housekeeping sigma factor in Pseudomonas fluorescens CHA0 enhances antibiotic production and improves biocontrol abilities.
Périodique
Journal of Bacteriology
Auteur⸱e⸱s
Schnider U., Keel C., Blumer C., Troxler J., Défago G., Haas D.
ISSN
0021-9193 (Print)
ISSN-L
0021-9193
Statut éditorial
Publié
Date de publication
1995
Volume
177
Numéro
18
Pages
5387-5392
Langue
anglais
Résumé
Pseudomonas fluorescens CHA0 produces a variety of secondary metabolites, in particular the antibiotics pyoluteorin and 2,4-diacetylphloroglucinol, and protects various plants from diseases caused by soilborne pathogenic fungi. The rpoD gene encoding the housekeeping sigma factor sigma 70 of P. fluorescens was sequenced. The deduced RpoD protein showed 83% identity with RpoD of Pseudomonas aeruginosa and 67% identity with RpoD of Escherichia coli. Attempts to inactivate the single chromosomal rpoD gene of strain CHA0 were unsuccessful, indicating an essential role of this gene. When rpoD was carried by an IncP vector in strain CHA0, the production of both antibiotics was increased severalfold and, in parallel, protection of cucumber against disease caused by Pythium ultimum was improved, in comparison with strain CHA0.
Mots-clé
Amino Acid Sequence, Anti-Bacterial Agents/biosynthesis, Antifungal Agents/biosynthesis, Bacterial Proteins/biosynthesis, Bacterial Proteins/genetics, Base Sequence, Cucumis sativus/microbiology, DNA-Directed RNA Polymerases/biosynthesis, DNA-Directed RNA Polymerases/genetics, Gene Amplification/genetics, Genes, Bacterial/genetics, Molecular Sequence Data, Phenols, Phloroglucinol/analogs & derivatives, Phloroglucinol/metabolism, Plant Roots/microbiology, Pseudomonas fluorescens/genetics, Pseudomonas fluorescens/metabolism, Pyrroles, Pythium/physiology, Restriction Mapping, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sigma Factor/biosynthesis, Sigma Factor/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 13:51
Dernière modification de la notice
20/08/2019 12:40
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