The nitric oxide pathway in pig isolated calyceal smooth muscle.

Details

Serval ID
serval:BIB_10825
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The nitric oxide pathway in pig isolated calyceal smooth muscle.
Journal
Neurourology and Urodynamics
Author(s)
Iselin C.E., Ny L., Mastrangelo D., Felley-Bosco E., Larsson B., Alm P., Andersson K.E.
ISSN
0733-2467 (Print)
ISSN-L
0733-2467
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
18
Number
6
Pages
673-685
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Abstract
In pig and humans, whose kidneys have a multi-calyceal collecting system, the initiation of ureteral peristalsis takes place in the renal calyces. In the pig and human ureter, recent evidence suggests that nitric oxide (NO) is an inhibitory mediator that may be involved in the regulation of peristalsis. This study was designed to assess whether the NO synthase/NO/cyclic GMP pathway modulates the motility of pig isolated calyceal smooth muscle. Immunohistochemistry revealed a moderate overall innervation of the smooth muscle layer, and no neuronal or inducible NO synthase (NOS) immunoreactivities. Endothelial NOS immunoreactivities were observed in the urothelium and vascular endothelium, and numerous cyclic GMP-immunoreactive (-IR) calyceal smooth muscle cells were found. As measured by monitoring the conversion of L-arginine to L-citrulline, Ca(2+)-dependent NOS activity was moderate. Assessment of functional effects was performed in tissue baths and showed that NO and SIN-1 decreased spontaneous and induced contractions of isolated preparations in a concentration-dependent manner. In strips exposed to NO, there was a 10-fold increase of the cyclic GMP levels compared with control preparations (P < 0.01). It is concluded that a non-neuronal NOS/NO/cyclic GMP pathway is present in pig calyces, where it may influence motility. The demonstration of cyclic GMP-IR smooth muscle cells suggests that NO acts directly on these cells. This NOS/NO/cyclic GMP pathway may be a target for drugs inhibiting peristalsis of mammalian upper urinary tract. Neurourol. Urodynam. 18:673-685, 1999.
Keywords
Animals, Humans, Kidney Calices/metabolism, Kidney Calices/physiopathology, Muscle Contraction/physiology, Muscle, Smooth/metabolism, Muscle, Smooth/physiopathology, Nitric Oxide/physiology, Signal Transduction, Swine
Pubmed
Web of science
Create date
19/11/2007 12:01
Last modification date
20/08/2019 12:37
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