The nitric oxide pathway in pig isolated calyceal smooth muscle.
Détails
ID Serval
serval:BIB_10825
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The nitric oxide pathway in pig isolated calyceal smooth muscle.
Périodique
Neurourology and Urodynamics
ISSN
0733-2467 (Print)
ISSN-L
0733-2467
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
18
Numéro
6
Pages
673-685
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Résumé
In pig and humans, whose kidneys have a multi-calyceal collecting system, the initiation of ureteral peristalsis takes place in the renal calyces. In the pig and human ureter, recent evidence suggests that nitric oxide (NO) is an inhibitory mediator that may be involved in the regulation of peristalsis. This study was designed to assess whether the NO synthase/NO/cyclic GMP pathway modulates the motility of pig isolated calyceal smooth muscle. Immunohistochemistry revealed a moderate overall innervation of the smooth muscle layer, and no neuronal or inducible NO synthase (NOS) immunoreactivities. Endothelial NOS immunoreactivities were observed in the urothelium and vascular endothelium, and numerous cyclic GMP-immunoreactive (-IR) calyceal smooth muscle cells were found. As measured by monitoring the conversion of L-arginine to L-citrulline, Ca(2+)-dependent NOS activity was moderate. Assessment of functional effects was performed in tissue baths and showed that NO and SIN-1 decreased spontaneous and induced contractions of isolated preparations in a concentration-dependent manner. In strips exposed to NO, there was a 10-fold increase of the cyclic GMP levels compared with control preparations (P < 0.01). It is concluded that a non-neuronal NOS/NO/cyclic GMP pathway is present in pig calyces, where it may influence motility. The demonstration of cyclic GMP-IR smooth muscle cells suggests that NO acts directly on these cells. This NOS/NO/cyclic GMP pathway may be a target for drugs inhibiting peristalsis of mammalian upper urinary tract. Neurourol. Urodynam. 18:673-685, 1999.
Mots-clé
Animals, Humans, Kidney Calices/metabolism, Kidney Calices/physiopathology, Muscle Contraction/physiology, Muscle, Smooth/metabolism, Muscle, Smooth/physiopathology, Nitric Oxide/physiology, Signal Transduction, Swine
OAI-PMH
Pubmed
Web of science
Création de la notice
19/11/2007 13:01
Dernière modification de la notice
20/08/2019 13:37