Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion.

Détails

Ressource 1Télécharger: Supplemental_Data.pdf (2144.62 [Ko])
Etat: Public
Version: Final published version
Document(s) secondaire(s)
Télécharger: Supplemental_Data.pdf (4277.74 [Ko])
Etat: Public
Version: Supplementary document
ID Serval
serval:BIB_0017F15B0B98
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion.
Périodique
Genes and Development
Auteur(s)
Dudin O., Merlini L., Martin S.G.
ISSN
0890-9369 (Print)
ISSN-L
0890-9369
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
30
Numéro
19
Pages
2226-2239
Langue
anglais
Résumé
Cell fusion is universal in eukaryotes for fertilization and development, but what signals this process is unknown. Here, we show in Schizosaccharomyces pombe that fusion does not require a dedicated signal but is triggered by spatial focalization of the same pheromone-GPCR (G-protein-coupled receptor)-MAPK signaling cascade that drives earlier mating events. Autocrine cells expressing the receptor for their own pheromone trigger fusion attempts independently of cell-cell contact by concentrating pheromone release at the fusion focus, a dynamic actin aster underlying the secretion of cell wall hydrolases. Pheromone receptor and MAPK cascade are similarly enriched at the fusion focus, concomitant with fusion commitment in wild-type mating pairs. This focalization promotes cell fusion by immobilizing the fusion focus, thus driving local cell wall dissolution. We propose that fusion commitment is imposed by a local increase in MAPK concentration at the fusion focus, driven by a positive feedback between fusion focus formation and focalization of pheromone release and perception.

Mots-clé
G-protein-coupled receptor signaling, MAP kinase cascade, cell–cell fusion, fertilization, fission yeast Schizosaccharomyces pombe, formin, pheromone
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/11/2016 16:06
Dernière modification de la notice
20/08/2019 13:22
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