Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.

Détails

ID Serval
serval:BIB_A63217826FFF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.
Périodique
Journal of Clinical Microbiology
Auteur(s)
Gayet-Ageron A., Laurent F., Schrenzel J., Charton B., Jimenez-Getaz G., Tangomo M., Ferry T., Sednaoui P., Lautenschlager S., Toutous-Trellu L., Martinez de Tejada B., Cavassini M., Emonet S., Perneger T., Salord H.
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
53
Numéro
3
Pages
976-980
Langue
anglais
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers using Tp-PCR targeting the tpp47 and then polA genes. The two methods showed similar accuracies and an almost-perfect agreement.
Mots-clé
Adult, Carrier Proteins/analysis, Carrier Proteins/genetics, DNA Polymerase I/analysis, DNA Polymerase I/genetics, Female, Humans, Lipoproteins/analysis, Lipoproteins/genetics, Male, Middle Aged, Molecular Diagnostic Techniques/methods, Polymerase Chain Reaction/methods, Prospective Studies, Syphilis/diagnosis, Syphilis/microbiology, Treponema pallidum/genetics, Treponema pallidum/isolation & purification, Ulcer/microbiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
26/03/2015 19:23
Dernière modification de la notice
20/08/2019 16:11
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