Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.

Details

Serval ID
serval:BIB_A63217826FFF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.
Journal
Journal of Clinical Microbiology
Author(s)
Gayet-Ageron A., Laurent F., Schrenzel J., Charton B., Jimenez-Getaz G., Tangomo M., Ferry T., Sednaoui P., Lautenschlager S., Toutous-Trellu L., Martinez de Tejada B., Cavassini M., Emonet S., Perneger T., Salord H.
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
53
Number
3
Pages
976-980
Language
english
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers using Tp-PCR targeting the tpp47 and then polA genes. The two methods showed similar accuracies and an almost-perfect agreement.
Keywords
Adult, Carrier Proteins/analysis, Carrier Proteins/genetics, DNA Polymerase I/analysis, DNA Polymerase I/genetics, Female, Humans, Lipoproteins/analysis, Lipoproteins/genetics, Male, Middle Aged, Molecular Diagnostic Techniques/methods, Polymerase Chain Reaction/methods, Prospective Studies, Syphilis/diagnosis, Syphilis/microbiology, Treponema pallidum/genetics, Treponema pallidum/isolation & purification, Ulcer/microbiology
Pubmed
Web of science
Open Access
Yes
Create date
26/03/2015 18:23
Last modification date
20/08/2019 15:11
Usage data