Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.

Gayet-Ageron, A.; Laurent, F.; Schrenzel, J.; Charton, B.; Jimenez-Getaz, G.; Tangomo, M.; Ferry, T.; Sednaoui, P.; Lautenschlager, S.; Toutous-Trellu, L.; Martinez de Tejada, B.; Cavassini, M.; Emonet, S.; Perneger, T.; Salord, H.

doi:10.1128/JCM.03444-14

Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.

Details

Request a copy

Serval ID

serval:BIB_A63217826FFF

Type

Article: article from journal or magazin.

Collection

Publications

Institution

UNIL/CHUV

Title

Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.

Journal

Journal of Clinical Microbiology

Author(s)

Gayet-Ageron A., Laurent F., Schrenzel J., Charton B., Jimenez-Getaz G., Tangomo M., Ferry T., Sednaoui P., Lautenschlager S., Toutous-Trellu L., Martinez de Tejada B., Cavassini M., Emonet S., Perneger T., Salord H.

ISSN

1098-660X (Electronic)

ISSN-L

0095-1137

Publication state

Published

Issued date

2015

Peer-reviewed

Oui

Volume

Number

Pages

976-980

Language

english

Notes

Publication types: Comparative Study ; Evaluation Studies ; Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
Publication Status: ppublish

Abstract

Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers using Tp-PCR targeting the tpp47 and then polA genes. The two methods showed similar accuracies and an almost-perfect agreement.

Keywords

Adult, Carrier Proteins/analysis, Carrier Proteins/genetics, DNA Polymerase I/analysis, DNA Polymerase I/genetics, Female, Humans, Lipoproteins/analysis, Lipoproteins/genetics, Male, Middle Aged, Molecular Diagnostic Techniques/methods, Polymerase Chain Reaction/methods, Prospective Studies, Syphilis/diagnosis, Syphilis/microbiology, Treponema pallidum/genetics, Treponema pallidum/isolation & purification, Ulcer/microbiology

OAI-PMH

oai:serval.unil.ch:BIB_A63217826FFF

DOI

10.1128/JCM.03444-14

Pubmed

25520453

Web of science

000350204600034

Open Access

Yes

Create date

26/03/2015 19:23