Isocitrate lyase from germinating Soybean cotyledons: Purification and characterization
Détails
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Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_6999A06A8D7C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Isocitrate lyase from germinating Soybean cotyledons: Purification and characterization
Périodique
Journal of Experimental Botany
ISSN
0022-0957
Statut éditorial
Publié
Date de publication
1986
Peer-reviewed
Oui
Volume
37
Numéro
11
Pages
1685-1690
Langue
anglais
Résumé
Isocitrate lyase (E.C. 4.1.3.1) was purified from the cotyledons of 7-d-old soybean seedlings. Three molecular forms were detected with pi values of 6·46, 6·25 and 6·0. The main form (pl = 6·46) had an approximate Mr of 130 000, a pH optimum of 8·0, a Km (isocitrate) close to 2·0 mol m-3 and a molecular activity of 615 min-1 at 25 °C. The purified enzyme is not a glycoprotein and is heat labile.
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13/08/2015 8:01
Dernière modification de la notice
20/08/2019 14:24
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