Isocitrate lyase from germinating Soybean cotyledons: Purification and characterization

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State: Public
Version: author
Serval ID
serval:BIB_6999A06A8D7C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
UNIL/CHUV
Title
Isocitrate lyase from germinating Soybean cotyledons: Purification and characterization
Journal
Journal of Experimental Botany
Author(s)
Ruchti M., Widmer F.
ISSN
0022-0957
Publication state
Published
Issued date
1986
Peer-reviewed
Oui
Volume
37
Number
11
Pages
1685-1690
Language
english
Abstract
Isocitrate lyase (E.C. 4.1.3.1) was purified from the cotyledons of 7-d-old soybean seedlings. Three molecular forms were detected with pi values of 6·46, 6·25 and 6·0. The main form (pl = 6·46) had an approximate Mr of 130 000, a pH optimum of 8·0, a Km (isocitrate) close to 2·0 mol m-3 and a molecular activity of 615 min-1 at 25 °C. The purified enzyme is not a glycoprotein and is heat labile.
OAI-PMH
oai:serval.unil.ch:BIB_6999A06A8D7C
DOI
10.1093/jxb/37.11.1685
Web of science
A1986F030500009
Create date
13/08/2015 8:01
Last modification date
20/08/2019 14:24
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