Comprehensive proteomic analysis of JC polyomavirus-infected human astrocytes and their extracellular vesicles.
Détails
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_5E6A065E798E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comprehensive proteomic analysis of JC polyomavirus-infected human astrocytes and their extracellular vesicles.
Périodique
Microbiology spectrum
ISSN
2165-0497 (Electronic)
ISSN-L
2165-0497
Statut éditorial
Publié
Date de publication
12/12/2023
Peer-reviewed
Oui
Volume
11
Numéro
6
Pages
e0275123
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Progressive multifocal leukoencephalopathy is a crimpling demyelinating disease of the central nervous system caused by JC polyomavirus (JCPyV). Much about JCPyV propagation in the brain remains obscure because of a lack of proper animal models to study the virus in the context of the disease, thus hampering efforts toward the development of new antiviral strategies. Here, having established a robust and representative model of JCPyV infection in human-induced pluripotent stem cell-derived astrocytes, we are able to fully characterize the effect of JCPyV on the biology of the cells and show that the proteomic signature observed for JCPyV-infected astrocytes is extended to extracellular vesicles (EVs). These data suggest that astrocyte-derived EVs found in body fluids might serve as a rich source of information relevant to JCPyV infection in the brain, opening avenues toward better understanding the pathogenesis of the virus and, ultimately, the identification of new antiviral targets.
Mots-clé
Animals, Humans, JC Virus/physiology, Astrocytes, Proteomics, Polyomavirus Infections, Extracellular Vesicles, Antiviral Agents, JCPyV, PML, astrocytes, extracellular vesicles, hiPSC, model
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/10/2023 9:45
Dernière modification de la notice
08/08/2024 7:34