Detection of enteroviruses in the cerebrospinal fluid by polymerase chain reaction: prospective study of impact on the management of hospitalized children

Détails

ID Serval
serval:BIB_5B62DB389217
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Detection of enteroviruses in the cerebrospinal fluid by polymerase chain reaction: prospective study of impact on the management of hospitalized children
Périodique
Clinical Pediatrics
Auteur⸱e⸱s
Spicher  V. M., Berclaz  P. Y., Cheseaux  J. J., Morandi  P. A., Suter  S., Wunderli  W., Siegrist  C. A.
ISSN
0009-9228 (Print)
Statut éditorial
Publié
Date de publication
04/2000
Volume
39
Numéro
4
Pages
203-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
A polymerase chain reaction kit (AMPLICOR EV) for the detection of enteroviruses (EV-PCR) in the cerebrospinal fluid (CSF) was evaluated in clinical conditions in a prospective blinded-intention study. Forty-three children (mean age 2.7 years) hospitalized for suspected meningitis or fever of unclear etiology were enrolled. EV-PCR was performed on a daily basis. Results were available in less than 2 days in 72% of cases. EV-PCR was positive in nine (21%) children, including three infants without CSF pleocytosis. Knowing their EV-PCR result would have allowed a saving of 18 hospital days and 12 days of antibiotic therapy. The EV-PCR in the CSF can thus be practically useful for children hospitalized for meningitis or fever if available on-site on a daily basis.
Mots-clé
Adolescent Cerebrospinal Fluid/virology Child Child, Preschool Diagnosis, Differential Enterovirus Infections/*cerebrospinal fluid Evaluation Studies Female Fever of Unknown Origin/cerebrospinal fluid/microbiology Humans Infant Infant, Newborn Length of Stay Male Meningitis, Viral/cerebrospinal fluid/microbiology Polymerase Chain Reaction/*methods Prospective Studies
Pubmed
Web of science
Création de la notice
25/01/2008 10:04
Dernière modification de la notice
20/08/2019 14:14
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