Detection of enteroviruses in the cerebrospinal fluid by polymerase chain reaction: prospective study of impact on the management of hospitalized children
Details
Serval ID
serval:BIB_5B62DB389217
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detection of enteroviruses in the cerebrospinal fluid by polymerase chain reaction: prospective study of impact on the management of hospitalized children
Journal
Clinical Pediatrics
ISSN
0009-9228 (Print)
Publication state
Published
Issued date
04/2000
Volume
39
Number
4
Pages
203-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
A polymerase chain reaction kit (AMPLICOR EV) for the detection of enteroviruses (EV-PCR) in the cerebrospinal fluid (CSF) was evaluated in clinical conditions in a prospective blinded-intention study. Forty-three children (mean age 2.7 years) hospitalized for suspected meningitis or fever of unclear etiology were enrolled. EV-PCR was performed on a daily basis. Results were available in less than 2 days in 72% of cases. EV-PCR was positive in nine (21%) children, including three infants without CSF pleocytosis. Knowing their EV-PCR result would have allowed a saving of 18 hospital days and 12 days of antibiotic therapy. The EV-PCR in the CSF can thus be practically useful for children hospitalized for meningitis or fever if available on-site on a daily basis.
Keywords
Adolescent
Cerebrospinal Fluid/virology
Child
Child, Preschool
Diagnosis, Differential
Enterovirus Infections/*cerebrospinal fluid
Evaluation Studies
Female
Fever of Unknown Origin/cerebrospinal fluid/microbiology
Humans
Infant
Infant, Newborn
Length of Stay
Male
Meningitis, Viral/cerebrospinal fluid/microbiology
Polymerase Chain Reaction/*methods
Prospective Studies
Pubmed
Web of science
Create date
25/01/2008 11:04
Last modification date
20/08/2019 15:14