Detection of enteroviruses in the cerebrospinal fluid by polymerase chain reaction: prospective study of impact on the management of hospitalized children

Details

Serval ID
serval:BIB_5B62DB389217
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detection of enteroviruses in the cerebrospinal fluid by polymerase chain reaction: prospective study of impact on the management of hospitalized children
Journal
Clinical Pediatrics
Author(s)
Spicher  V. M., Berclaz  P. Y., Cheseaux  J. J., Morandi  P. A., Suter  S., Wunderli  W., Siegrist  C. A.
ISSN
0009-9228 (Print)
Publication state
Published
Issued date
04/2000
Volume
39
Number
4
Pages
203-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
A polymerase chain reaction kit (AMPLICOR EV) for the detection of enteroviruses (EV-PCR) in the cerebrospinal fluid (CSF) was evaluated in clinical conditions in a prospective blinded-intention study. Forty-three children (mean age 2.7 years) hospitalized for suspected meningitis or fever of unclear etiology were enrolled. EV-PCR was performed on a daily basis. Results were available in less than 2 days in 72% of cases. EV-PCR was positive in nine (21%) children, including three infants without CSF pleocytosis. Knowing their EV-PCR result would have allowed a saving of 18 hospital days and 12 days of antibiotic therapy. The EV-PCR in the CSF can thus be practically useful for children hospitalized for meningitis or fever if available on-site on a daily basis.
Keywords
Adolescent Cerebrospinal Fluid/virology Child Child, Preschool Diagnosis, Differential Enterovirus Infections/*cerebrospinal fluid Evaluation Studies Female Fever of Unknown Origin/cerebrospinal fluid/microbiology Humans Infant Infant, Newborn Length of Stay Male Meningitis, Viral/cerebrospinal fluid/microbiology Polymerase Chain Reaction/*methods Prospective Studies
Pubmed
Web of science
Create date
25/01/2008 10:04
Last modification date
20/08/2019 14:14
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