A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.

Détails

ID Serval
serval:BIB_37FE0AC1DE8A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.
Périodique
Journal of immunological methods
Auteur(s)
Peitsch M.C., Kovacsovics T.J., Isliker H.
ISSN
0022-1759 (Print)
ISSN-L
0022-1759
Statut éditorial
Publié
Date de publication
06/04/1988
Peer-reviewed
Oui
Volume
108
Numéro
1-2
Pages
265-269
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents.
Mots-clé
Chromatography, High Pressure Liquid/methods, Complement Activating Enzymes/immunology, Complement Activating Enzymes/isolation & purification, Complement Activating Enzymes/metabolism, Complement C1/immunology, Complement C1/isolation & purification, Complement C1/metabolism, Complement C1r, Complement C1s/immunology, Complement C1s/isolation & purification, Complement C1s/metabolism, Complement Pathway, Classical, Enzyme Activation, Enzyme Precursors/immunology, Enzyme Precursors/isolation & purification, Enzyme Precursors/metabolism, Humans
Pubmed
Web of science
Création de la notice
06/08/2018 11:35
Dernière modification de la notice
20/08/2019 14:26
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