A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.
Details
Serval ID
serval:BIB_37FE0AC1DE8A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.
Journal
Journal of immunological methods
ISSN
0022-1759 (Print)
ISSN-L
0022-1759
Publication state
Published
Issued date
06/04/1988
Peer-reviewed
Oui
Volume
108
Number
1-2
Pages
265-269
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents.
Keywords
Chromatography, High Pressure Liquid/methods, Complement Activating Enzymes/immunology, Complement Activating Enzymes/isolation & purification, Complement Activating Enzymes/metabolism, Complement C1/immunology, Complement C1/isolation & purification, Complement C1/metabolism, Complement C1r, Complement C1s/immunology, Complement C1s/isolation & purification, Complement C1s/metabolism, Complement Pathway, Classical, Enzyme Activation, Enzyme Precursors/immunology, Enzyme Precursors/isolation & purification, Enzyme Precursors/metabolism, Humans
Pubmed
Web of science
Create date
06/08/2018 11:35
Last modification date
20/08/2019 14:26
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