A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.

Details

Serval ID
serval:BIB_37FE0AC1DE8A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography.
Journal
Journal of immunological methods
Author(s)
Peitsch M.C., Kovacsovics T.J., Isliker H.
ISSN
0022-1759 (Print)
ISSN-L
0022-1759
Publication state
Published
Issued date
06/04/1988
Peer-reviewed
Oui
Volume
108
Number
1-2
Pages
265-269
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents.
Keywords
Chromatography, High Pressure Liquid/methods, Complement Activating Enzymes/immunology, Complement Activating Enzymes/isolation & purification, Complement Activating Enzymes/metabolism, Complement C1/immunology, Complement C1/isolation & purification, Complement C1/metabolism, Complement C1r, Complement C1s/immunology, Complement C1s/isolation & purification, Complement C1s/metabolism, Complement Pathway, Classical, Enzyme Activation, Enzyme Precursors/immunology, Enzyme Precursors/isolation & purification, Enzyme Precursors/metabolism, Humans
Pubmed
Web of science
Create date
06/08/2018 10:35
Last modification date
20/08/2019 13:26
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