TLR5 signaling stimulates the innate production of IL-17 and IL-22 by CD3(neg)CD127+ immune cells in spleen and mucosa.

Details

Serval ID
serval:BIB_FDDFF725E09A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
TLR5 signaling stimulates the innate production of IL-17 and IL-22 by CD3(neg)CD127+ immune cells in spleen and mucosa.
Journal
Journal of Immunology
Author(s)
Van Maele L., Carnoy C., Cayet D., Songhet P., Dumoutier L., Ferrero I., Janot L., Erard F., Bertout J., Leger H., Sebbane F., Benecke A., Renauld J.C., Hardt W.D., Ryffel B., Sirard J.C.
ISSN
1550-6606[electronic], 0022-1767[linking]
Publication state
Published
Issued date
2010
Volume
185
Number
2
Pages
1177-1185
Language
english
Abstract
In adaptive immunity, Th17 lymphocytes produce the IL-17 and IL-22 cytokines that stimulate mucosal antimicrobial defenses and tissue repair. In this study, we observed that the TLR5 agonist flagellin induced swift and transient transcription of genes encoding IL-17 and IL-22 in lymphoid, gut, and lung tissues. This innate response also temporarily enhanced the expression of genes associated with the antimicrobial Th17 signature. The source of the Th17-related cytokines was identified as novel populations of CD3(neg)CD127(+) immune cells among which CD4-expressing cells resembling lymphoid tissue inducer cells. We also demonstrated that dendritic cells are essential for expression of Th17-related cytokines and so for stimulation of innate cells. These data define that TLR-induced activation of CD3(neg)CD127(+) cells and production of Th17-related cytokines may be crucial for the early defenses against pathogen invasion of host tissues.
Keywords
Animals, Antigens, CD3/genetics, Antigens, CD3/immunology, Cells, Cultured, Dendritic Cells/drug effects, Dendritic Cells/immunology, Female, Flagellin/pharmacology, Flow Cytometry, Gene Expression/drug effects, Gene Expression/immunology, Ileum/drug effects, Ileum/immunology, Interleukin-17/genetics, Interleukin-17/immunology, Interleukin-7 Receptor alpha Subunit/genetics, Interleukin-7 Receptor alpha Subunit/immunology, Interleukins/genetics, Interleukins/immunology, Lymphoid Tissue/cytology, Lymphoid Tissue/immunology, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Mice, SCID, Mice, Transgenic, Mucous Membrane/cytology, Mucous Membrane/immunology, Oligonucleotide Array Sequence Analysis, Signal Transduction/immunology, Spleen/cytology, Spleen/immunology, Toll-Like Receptor 5/genetics, Toll-Like Receptor 5/immunology
Pubmed
Web of science
Open Access
Yes
Create date
02/02/2011 10:25
Last modification date
20/08/2019 16:28
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