Local shockwave-application prevents flap necrosis

Details

Serval ID
serval:BIB_FB72AACDFE31
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Local shockwave-application prevents flap necrosis
Title of the conference
ESSR 2010, 45th Congress of the European Society for Surgical Research
Author(s)
Harder Y., Vigato E., Pittet B., Wettstein R., Rezaeian F., Tobalem M.
Address
Geneva, Switzerland, June 9-12, 2010
ISBN
0007-1323
Publication state
Published
Issued date
2010
Volume
97
Series
British Journal of Surgery
Pages
S16
Language
english
Notes
Meeting Abstract
Abstract
Objective:
Local shockwave-application (SW) has shown to improve healing
of various tissues and decrease necrosis of flaps. Though, there is no data about
the optimal time-point of SW-application with regard to induction of ischemia
(i.e. flap elevation) and subsequent effect on flap survival. Therefore we compared
2 shock-wave protocols in a model of persistent ischemia and investigated
underlying mechanisms.
Methods:
18 C57BL/6-mice equipped with a skinfold chamber containing a
musculocutaneous flap were assigned to 3 experimental groups: 1. One session
of 500 SWimpulses at 0·15 mJ/mm2 applied 24 hrs before (preconditioning) or
2. Applied 30 min after flap elevation (treatment). 3. Untreated flaps (control).
Tissue necrosis,microhemodynamics, inflammation, apoptosis and angiogenesis
were assessed by intravital epi-fluorescence microscopy over 10 days.
Results:
SW significantly reduced flap necrosis independent from the
application time-point (preconditioning: 29 ± 7%; treatment: 25 ± 7% vs.
control: 47 ± 2%; d10, p<0·05). This was associated with an early increase
of functional capillary density (preconditioning: 236 ± 39 cm/cm2; treatment:
211 ± 33 cm/cm2 vs. control: 141 ± 7 cm/cm2; day1, p<0·05). Arteriolar diameter,
red blood cell velocity and blood flow were comparable between the
3 experimental groups. SW-application significantly decreased the ischemiainduced
inflammatory response (apoptotic cell death and leukocyte-endothelial
interaction: (p<0·05)). Sprouts indicating angiogenesis were observed from day
7 only after SW-application.
Conclusions:
SW protects ischemically challenged musculocutaneous tissue.
Interestingly, postoperative SW-application is as efficient as preoperative SWapplication.
The protective effect induced by mechanical stress might be based
on an early recruitment of ''sleeping capillaries'' maintaining nutritive perfusion
and an anti-inflammatory effect within the ischemically jeopardized tissue. SWapplication
provides a non-invasive alternative to local thermic and systemic
pre-treatment of endangered tissues.
Web of science
Create date
21/10/2010 10:39
Last modification date
20/08/2019 16:26
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