A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis
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State: Public
Version: Final published version
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
State: Public
Version: Final published version
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_EEBF69E055C2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis
Journal
FEMS Microbiology Letters
ISSN
0378-1097 (Print)
Publication state
Published
Issued date
10/2000
Volume
191
Number
2
Pages
199-203
Notes
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Abstract
Borrelia garinii is one of the three major Borreliae responsible for Lyme borreliosis in Europe. We have characterized a protein of B. garinii (VS102) and a genomic fragment from the gene encoding this protein was cloned. The DNA sequence of the fragment showed high homology with a known gene of B. burgdorferi sensu stricto. The protein encoded by this gene in B. burgdorferi sensu stricto is a phosphocarrier protein (histidine-containing protein). A mutation T to G polymorphism at codon 57 was found to be specific to B. garinii. A PCR-based approach that allows the rapid detection of this mutation made it possible to specifically discriminate B. garinii from other B. burgdorferi genospecies with high sensitivity and specificity.
Keywords
Animals
*Bacterial Proteins
Base Sequence
Borrelia/*classification/genetics/isolation & purification
Cloning, Molecular
Genes, Bacterial
Humans
Lyme Disease/*microbiology
Molecular Sequence Data
Mutation
Phosphoenolpyruvate Sugar Phosphotransferase System/*genetics
Polymerase Chain Reaction/*methods
Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 21:51
Last modification date
14/02/2022 8:57