A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis

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Serval ID
serval:BIB_EEBF69E055C2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis
Journal
FEMS Microbiology Letters
Author(s)
Mueller  C., Hutter  P., Sahli  R., Bretz  A., Toutoungi  L. N., Peter  O.
ISSN
0378-1097 (Print)
Publication state
Published
Issued date
10/2000
Volume
191
Number
2
Pages
199-203
Notes
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Abstract
Borrelia garinii is one of the three major Borreliae responsible for Lyme borreliosis in Europe. We have characterized a protein of B. garinii (VS102) and a genomic fragment from the gene encoding this protein was cloned. The DNA sequence of the fragment showed high homology with a known gene of B. burgdorferi sensu stricto. The protein encoded by this gene in B. burgdorferi sensu stricto is a phosphocarrier protein (histidine-containing protein). A mutation T to G polymorphism at codon 57 was found to be specific to B. garinii. A PCR-based approach that allows the rapid detection of this mutation made it possible to specifically discriminate B. garinii from other B. burgdorferi genospecies with high sensitivity and specificity.
Keywords
Animals *Bacterial Proteins Base Sequence Borrelia/*classification/genetics/isolation & purification Cloning, Molecular Genes, Bacterial Humans Lyme Disease/*microbiology Molecular Sequence Data Mutation Phosphoenolpyruvate Sugar Phosphotransferase System/*genetics Polymerase Chain Reaction/*methods Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 21:51
Last modification date
25/09/2019 7:11
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