Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_E29E6F7F6BE0
Type
Article: article from journal or magazin.
Collection
Publications
Title
Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.
Journal
Cell
Author(s)
Gouge J., Satia K., Guthertz N., Widya M., Thompson A.J., Cousin P., Dergai O., Hernandez N., Vannini A.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Publication state
Published
Issued date
2015
Volume
163
Number
6
Pages
1375-1387
Language
english
Abstract
TFIIB-related factor 2 (Brf2) is a member of the family of TFIIB-like core transcription factors. Brf2 recruits RNA polymerase (Pol) III to type III gene-external promoters, including the U6 spliceosomal RNA and selenocysteine tRNA genes. Found only in vertebrates, Brf2 has been linked to tumorigenesis but the underlying mechanisms remain elusive. We have solved crystal structures of a human Brf2-TBP complex bound to natural promoters, obtaining a detailed view of the molecular interactions occurring at Brf2-dependent Pol III promoters and highlighting the general structural and functional conservation of human Pol II and Pol III pre-initiation complexes. Surprisingly, our structural and functional studies unravel a Brf2 redox-sensing module capable of specifically regulating Pol III transcriptional output in living cells. Furthermore, we establish Brf2 as a central redox-sensing transcription factor involved in the oxidative stress pathway and provide a mechanistic model for Brf2 genetic activation in lung and breast cancer.
Pubmed
Web of science
Open Access
Yes
Create date
04/01/2016 10:59
Last modification date
20/08/2019 17:06
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